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Protein Folding Under Cellular Crowding

Simulating how macromolecular crowding reshapes folding landscapes, reconciling in-vitro kinetics with the dense reality of the living cell.

PI · Dr. Felix MwangiDr. Felix Mwangi

Objectives

  • Parameterize crowding effects in coarse-grained models
  • Validate against single-molecule FRET data
  • Identify crowding-sensitive folding intermediates

Methodology

Massively parallel molecular dynamics with implicit-crowder potentials, cross-validated against published single-molecule fluorescence trajectories.